ASSESSMENT OF CELLULASE COMPLEX SECRETORY CAPACITY OF TRICHODERMA STRAINS AND MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF THE ISOLATE WITH THE HIGHEST ENZYMATIC SECRETION CAPACITY

The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use plant as energy. production cellulase main factor in cellulose hydrolysis. Among producers are filamentous fungi. Therefore, many efforts have been made obtaining new microorganisms with high secretion capacity. secretory capacity 28 isolates Trichoderma was qualitatively and quantitatively evaluated. detection cellulolytic fungi correlated both Congo red dinitrosalicylic acid reagent methods. Based on assays, sixteen revealed carboxymethyl degradation ability, where POS7 isolate showed highest increase filter paper activity, endo-1,4-β-glucanases β-glucosidases activities (p<0.05) short incubation time. This molecularly identified koningiopsis, based internal transcribed spacer sequences. Our results provide information reveal microorganism hydrolysis material. phylogenetic analysis close positioning T. koningiopsis clade viride, viridescens petersenii clades closely related group, concordance current taxonomic classification genus.